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It is a substrate used for fluorometric assay for β-galactosidase. It is used to detect E-coli n the fluorogenic medium.
Manafi M.; Kneifel W. A combined chromogenic-fluorogenic medium for the simultaneous detection of coliform groups and E. coli in water.europe Pubmed central. 1989, 189 (3),225-234 .
Takayuki Ezaki.;Yasuhiro Hashimoto.; Eiko Yabuuchi . Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains.international journal of systmatic and evolutionary microbiolgy. 1989, 39 (3),224-229 .